I have provided some info for this report plus the info regarding the first patient. I need someone who has experience in writing scientific reports and is familiar with lab results and how to analyse them and only then he or she will receive the rest of the info.
Please read carefully whether you understand the underneath info, otherwise it is a very straight forward task with no difficulties.
The results need to be considered in the context of the four sets of patient history on the following pages. In each case you are required to provide a preliminary diagnosis and assess the patient’s healthcare requirements including any further tests required to confirm diagnosis, appropriate treatment options and ‘life-style’ advice that might be dispensed.
In this method, different viral antigen extracts have been immobilised onto the surface of plastic wells in a microtitre plate. Diluted patients sera are adsorbed to the wells and the specific antiviral antibodies bind to the different antigens lining the wells. After a wash stage, to rid of unbound patient's antibody, an antihuman antibody conjugated to an enzyme is added. After incubation, the plates are again washed to free from unbound conjugate and a substrate added. After a few minutes incubation, the enzyme cleaves the colourless substrate to produce a yellow solution in the wells. The intensity of the colour can be estimated colorimetrically using an ELISA plate reader. Only those samples containing the specific antiviral antibodies will produce a colour reaction.
In this simple experiment, the wells have already been coated with the viral antigen extract. The four viral antigens are Measles, Mumps, Rubella and Cytomegalovirus. Four patients serum samples, P1 to P4 are provided, together with both positive and negative control sera. The positive serum is designed to produce a density of colour that acts as a cut-off for determining positive samples.
This practical should be written up as a ‘traditional’ practical report including an introduction, methods, results and discussion. The report should be relatively brief, report the results in a suitable format and the discussion etc. should focus on the ‘mechanistic’ aspects of performing the ELISA. This report should then be followed by a more in-depth interpretation of the results in conjunction with the case studies of the four patients
Patient 1, the headmaster of a large primary school, is a 56 year old male. He presented to the out-of-hours GP service at his local hospital on Sunday evening with dense reddish-brown maculopapular rash covering much of his neck, chest and back with a somewhat less dense coverage on his face and limbs. He reports that he has been feeling ill with a headache, cold-like symptoms and low grade fever for a few days and noticed a few spots on his chest when he went to bed Saturday night. When he awoke Sunday morning the rash was considerably more prominent and has become worse through the day. He also reports mild abdominal discomfort and diarrhœa for which he has taken Immodium.
Along with confirmation of the widespread rash which fades under pressure, physical examination revealed a temperature of 38.2ºC and the presence of mild conjunctivitis. Auscultation indicated no abnormal respiratory sounds.
The GP took a throat swab to send for microbial culture and a blood sample drawn by venepuncture into a tube containing no anticoagulant for an anti-viral antibody screen.
Culture of the throat swab revealed nothing of significance.